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This cross-section study was focused to the useful of the herbal plants by the people in Lomsak District, Phetchabun Province. This study explored on the knowledge of popular herbal usefulness. Questionnaire and interview was used to collect the data among population and rural herbalist. In addition the characteristic of radical scavenging activity in local herbs were investigated. This study divided into four parts.
This research studied on the availability of the herbal types and their application wisdoms on Thai traditional medicine in local community. The results found that the most of the interviewer who was a rural herbalist was a female with age more than 56 year old. Their religion were Buddhism. Almost of the interviewers were married and finished the below or primary school. Most of them can be reading and writing. The occupation of rural herbalists were a famer. The wisdom of herbal medicine discipline report to apply for treated for illness such as fever, stomachache, Thyroidism, heal a wound, Herpes zoster, spider venoms, restorative herbs and vomiting. The study on role and form of rural herbal specialist found that the reason to became a herbalist because of some people in theirs family was a rural herbal specialist. The wisdom knowledge derived from theirs relatives or ancestors and engraved on their memory. The treatment style was using rural herbs integrated with conventional medicines. The single herbal plant was used to treat the patient. And also the rural herbal specialist planted these herbs around theirs home.
The radical scavenging activity of herbs 8 kinds including Mollugo pentapuhylla L., Suregada multiflorum A.Juss., Bauhinia strychnifolia Craib., Geodorum attenuatum Griff, Hiptage candicans Hook.f., Olax psittacorum (Willd.) Vahl., Smilax glabra Roxb. and Tiliacora triandra (Colebr.) Diels. It was extract of 50% ethanol. The study an antioxidant activity was evaluated using 2,2-diphenyl-1-picryl-hydrazyl (DPPH) free radical method. Their antioxidant properties using Vitamin C and Trolox were used as reference standard. The results showed that B.strychnifolia extract possess the most potent properties, IC50 of water soluble fraction and water insoluble fraction were 57.89 µg/ml and 364.55 µg/ml, respectively. IC50 of vitamin C were 9.13 µg/ml whereas IC50 of Trolox 15.97 µg/ml. Phytochemical screening and TLC fingerprints of B. strychnifolia indicated the presence of flavonoid or flavonol-3-glycoside and hydrolysable tannin. But H. candicans, O. psittacorum and S. glabra contained flavanone and flavonol. H. candicans, S. glabra and T. triandra contained condensed tannin. TLC fingerprints indicated that M. pentapuhylla, S. multiflorum, B. strychnifolia, H. candicans , O. psittacorum, S. glabra. be compounded of tannic acid. Wheras catechol was a component of G. attenuatum.
The activities on promoted, conserved, valuable and usefulness of herbal plants was done by transferred the knowledge on the project entitle “Traditional medicine training on rural herbal plant in life for conservation” to public health volunteers. The objective of this project were to promoted the safe and appropriated treatment by traditional medicine and herbs. And also, the people can be integrate the knowledge to theirs normal life. The evaluation on traditional medicine training found that the volunteers considered at a good to very good level.
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| คำสำคัญ |
สมุนไพรท้องถิ่น,การแพทย์พื้นบ้าน,อนุมูลอิสระ,สารต้านอนุมูลอิสระ,การวิเคราะห์ฤทธิ์ต้านอนุมูลอิสระ,Thai local herb,Traditional medicine,Free radicals,antioxidants,antioxidant activity determination
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